Mass Cytometry as a Tool for Investigating Senescence in Multiple Model Systems

Cellular senescence is a durable cell cycle arrest as result of the finite proliferative capacity cells. Senescence responds to both intrinsic and extrinsic cellular stresses, such aging, mitochondrial dysfunction, irradiation, chemotherapy. Here, we report on use mass cytometry (MC) analyze multiple model systems demonstrate MC platform for analysis at single-cell level. We changes p16 expression, cycling fraction, histone tail modifications in several established senescent using isolated human T In bone marrow mesenchymal stromal cells (BMSCs), show increased expression with subsequent passage well reduction open chromatin marks. WI-38 cells, culture-induced oxidative stress-induced (OSIS). also Wanderlust, trajectory tool, how proteins. Finally, that repetitive stimulation CD3/CD28 beads induces an exhausted phenotype expression. This p16-expressing population exhibited higher exhaustion markers EOMES TOX. work demonstrates useful studying protein level, capable measuring once high confidence, thereby improving our understanding pathways.